Reconstitution & Dose Calculator

Step-by-step reconstitution

Bench-side walkthrough for a 5 mg vial reconstituted with 2 mL of bacteriostatic water.

1. Step 1

   Prepare the vial

   Let the sealed 5 mg lyophilised peptide vial reach room temperature. Wipe the rubber stopper with a fresh alcohol swab and let it dry for a few seconds.

2. Step 2

   Draw the bacteriostatic water

   Using a sterile syringe, draw exactly 2 mL (200 units on a U-100 syringe) of bacteriostatic water. Invert the water vial, keep the needle tip below the meniscus and tap out any air.

3. Step 3

   Add the diluent to the peptide

   Angle the needle so the stream runs down the inner glass wall of the peptide vial — never directly onto the powder. Empty the full 2 mL, then swirl gently until the solution is clear. Do not shake.

4. Step 4

   Confirm the resulting concentration

   5 mg ÷ 2 mL = 2500 mcg/mL. Label the vial with the concentration and today's date, and refrigerate between uses.

5. Step 5

   Draw the injection

   For a 250 mcg (0.25 mg) dose at 2500 mcg/mL, draw 0.1 mL — 10 units on a 1 mL (100u) insulin syringe.

6. Step 6

   Inject and log

   Rotate injection sites (typically subcutaneous into the abdominal fat, at least 5 cm from the umbilicus). At this dose the vial yields roughly 20 full doses before it needs to be replaced.

Educational reference only. Every peptide has its own reconstitution, storage and dosing profile — always cross-check against the product monograph and your prescribing clinician's guidance.

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